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Image Search Results
Journal: Hla
Article Title: Recombinant human monoclonal HLA antibodies of different IgG subclasses recognising the same epitope: Excellent tools to study differential effects of donor‐specific antibodies
doi: 10.1111/tan.13664
Figure Lengend Snippet: Recombinant IgG subclass HLA monoclonal antibodies (mAbs) could be detected by the corresponding IgG‐specific detection antibody. IgG subclass could be detected with IgG subclass enzyme‐linked immunosorbent assay (ELISA) kit for both WIM8E5rec‐IgG mAbs (A) and RLTK1E2rec‐IgG mAbs (B). Positive control is a human serum. (C) Similar findings were observed when screening recombinant WIM8E5 IgG subclass mAbs with Lifecodes Lifescreen Deluxe kit. The kit contains seven groups of HLA class I beads and each data point represents a single bead group. Kruskal‐Wallis test was used to compare median of all four detection antibodies per IgG subclass mAb. Error bars represent median ± interquartile range. MFI is mean fluorescence intensity. OD is optical density. *** P < .001, **** P < .0001
Article Snippet: The HLA specificities of the recombinant mAbs were determined by screening the recombinant mAbs with
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Positive Control, Fluorescence